Real-time observation of flow-induced cytoskeletal stress in living cells
نویسندگان
چکیده
منابع مشابه
Real-time observation of flow-induced cytoskeletal stress in living cells.
The mechanical stress due to shear flow has profound effects on cell proliferation, transport, gene expression, and apoptosis. The mechanisms for flow sensing and transduction are unclear, but it is postulated that fluid flow pulls upon the apical surface, and the resulting stress is eventually transmitted through the cytoskeleton to adhesion plaques on the basal surface. Here we report a direc...
متن کاملSpontaneous Mutation: Real-Time in Living Cells
A new study provides the first direct visualization of DNA replication errors as they become mutations in living cells. After decades of post-hoc inference of mutation rates and mechanisms, a new method opens the possibility of cell-, tissue- and cancer-clone-specific mutation-rate detection and real-time visualization of transient hypermutable states in situ.
متن کاملWatching real-time endocytosis in living cells
The precise sequence of events promoting clathrin-coated vesicle assembly is still debated. In this issue, Kadlecova et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608071) test structural models using quantitative microscopy in living cells to investigate the hierarchy and temporal importance of molecular events required for clathrin-coated pit initiation.
متن کاملQuantification of Cytoskeletal deformation in Living Cells
In biology, various cell types are sensitive to changes in mechanical loading. On the short term physiological mechanical loading leads to cell response. For instance upon increased load in the heart, muscle cells immediately increase their contractile force. In blood vessels, increased shear stress on endothelial cells leads to cellular deformation and subsequent vasodilation, mediated by chem...
متن کاملReal-time observation of polyelectrolyte-induced binding of charged bilayers.
We present real-time observations by confocal microscopy of the dynamic behavior of multilamellar vesicles (MLVs), composed of charged synthetic lipids, when put in contact with oppositely charged polyelectrolyte (PE) molecules. We find that the MLVs exhibit astonishing morphological transitions, which result from the discrete and progressive binding of the charged bilayers induced by a high PE...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: American Journal of Physiology-Cell Physiology
سال: 2011
ISSN: 0363-6143,1522-1563
DOI: 10.1152/ajpcell.00099.2011